human ec cell lines  (ATCC)

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) Kaplan-Meier plots showing that LARP1 overexpression is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort (n=454). (C) Immunoblot analysis illustrating the protein expression of LARP1 after transfecting ISHI cell line with control or LARP1 siRNA at 25, 50, 75, and 100 nM. β-actin was used as a loading control. (D) Images derived from immunofluorescence analysis showing the protein expression of LARP1 after transfecting HEC-1A cells with control or LARP1 siRNA at 75 µM. Scale bar = 50 µM.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Over Expression, Western Blot, Expressing, Control, Derivative Assay, Immunofluorescence

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A – D) Cell viability assay showing the effect of two different LARP1 siRNA (1 and 2) on the viability of ISHI (A, B) and HEC-1A (C, D) cell lines. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots. (E) Immunoblot analysis showing the protein expression of cleaved PARP1 and cleaved caspase 3 after transfection of ISHI and HEC-1A cells with control or LARP1 siRNAs. β-actin was used as a loading control.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Viability Assay, Western Blot, Expressing, Transfection, Control

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Staining, Transfection

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) MTS cell viability assay showing the effect of escalating concentration of carboplatin on the viability of ISHI (A) and HEC-1A cell lines. Sterile water was used as a vehicle control. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Viability Assay, Concentration Assay, Sterility, Control

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, C) Micrographs representing colony formation assay showing the effect of LARP1 transfection and carboplatin treatment on the clonogenic survival of ISHI (A) and HEC-1A (B) cell lines. (B, D) Quantitative analysis of clonogenic assay showing the effect of LARP1 knockdown and carboplatin treatment on the clonogenic survival of ISHI (B) and HEC-1A (D) cells. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Colony Assay, Transfection, Clonogenic Assay, Knockdown

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: GATA6 is a target gene of miR-196b. A : The nucleotide sequence of the miR-196b target site in GATA6 3′-UTR. Mut: contains a four-base mutation at the miR-196b target region. B : Double-luciferase reporter assays were used to detect the binding of miR-196b to the 3’-UTR region of GATA6. C - F : qPCR and WB were used to test the mRNA ( C , D ) and protein ( E , F ) levels of GATA6 in Ishikawa ( C , E ) and HEC-1 A ( D , F ) cells. (* P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Sequencing, Mutagenesis, Luciferase, Binding Assay

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: miR-196b reduces EC proliferation, migration, and invasion. A - B : qPCR detection of the expression of miR-196b in Ishikawa ( A ) and HEC-1 A ( B ) cells transfected with miR-196b mimics ( a ) or anti-miR-196b ( b ). C - D : CCK-8 assays were carried out to evaluate the proliferation capacity of Ishikawa ( C ) and HEC-1 A ( D ) cells transfected with miR-196b mimics ( a ) or anti-miR-196b ( b ). E-F: Colony formation assays were utilized to assess cell growth in Ishikawa ( E ) and HEC-1 A ( F ) cells. G - H : Wound healing assays were used to measure the migration ability in Ishikawa ( G ) and HEC-1 A ( H ) cells. I - J : Transwell assays were conducted to determine the invasion ability in Ishikawa ( I ) and HEC-1 A ( J ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Migration, Expressing, Transfection, CCK-8 Assay

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: Impact of miR-196b on the cell cycle. A and C : Flow cytometry was used to detect the cell cycle distribution in Ishikawa ( A ) and HEC-1 A ( C ) cells following miR-196b mimics ( a ) and anti-miR-196b ( b ) transfection. B and D : WB to detect cyclin-associated protein levels in Ishikawa ( B ) and HEC-1 A ( D ) cells following miR-196b mimics ( a ) and anti-miR-196b ( b ) transfection. (* P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Flow Cytometry, Transfection

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: miR-196b inhibits EC proliferation in vivo. A : Weight growth curve of nude mice injected with agomir-196b-treated HEC-1 A cells. B : Tumor xenografts ( a ), tumor growth curve ( b ), and tumor weight ( c ) of nude mice at the study’s conclusion. qPCR ( d ) and WB ( e ) were performed to test the expression of miR-196b ( d ) and GATA6 ( e ) in tumors. The H&E staining reveals the pathological characteristics of the tumors from nude mice ( f ). C : Expression of GATA6, VEGF, p-AKT, and p-ERK in tumors was tested by immunohistochemical. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: In Vivo, Injection, Expressing, Staining, Immunohistochemical staining

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: The miR-196b/GATA6 axis regulates EC proliferation, migration, and invasion. A - F : CCK8 ( A , B ), clone formation ( C , D ), and EdU assays ( E , F ) were used to detect the proliferation capacity of Ishikawa ( A , C , E ) and HEC-1 A ( B , D , F ) cells transfected with anti-miR-196b, siGATA6, and anti-miR-196b+siGATA6. G - J : Wound healing ( G , H ) and Transwell ( I , J ) assays assessing cell migration and invasion in Ishikawa ( G , I ) and HEC-1 A ( H , J ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Migration, Transfection

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: miR-196b regulates the EMT process and AKT/ERK signaling pathways via GATA6 in EC cells. A - B : IF staining of E-cadherin ( A ) and vimentin ( B ) in Ishikawa ( a ) and HEC-1 A ( b ) cells. C - D : WB analysis of EMT-related markers ( C ) and AKT and ERK pathway-related proteins ( D ) in Ishikawa ( a ) and HEC-1 A ( b ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Protein-Protein interactions, Staining

Journal: Biomedicines

Article Title: Targeting the FTO-ACSL4 Pathway: A Novel Mechanism for Sanguinarine Chloride-Induced Ferroptosis in Endometrial Cancer

doi: 10.3390/biomedicines14030608

Figure Lengend Snippet: S.C provokes ferroptosis in EC cells. ( A , B ) EC cells were treated with S.C (1.0 μM) for 48 h in the presence or absence of Fer-1 (2.0 μM) or DFO (5.0 μM), and cell viability was assessed by CCK-8 assay. ( C ) Intracellular MDA levels were quantified in EC cells treated with S.C or RSL3 (positive control) for 48 h using commercial assay kits. ( D , E ) WB analysis of protein levels of NCOA4, NRF2, ACSL4, KEAP1, SLC7A11, SLC11A2, SLC40A1, SCD, FTH, FTL, FSP1, and GPX4 in EC cells treated with S.C for 48 h. ( F , G ) Intracellular ROS levels were measured by flow cytometry using the fluorescent probe DCFH-DA (10 μM) in EC cells treated with S.C (1.0 μM) or without DFO (5.0 μM) for 48 h. ( H , I ) Lipid peroxidation was detected using C11-BODIPY 581/591 probe after HEC-1-A and AN3CA cells were treated with S.C for 48 h. Red fluorescence represents the reduction state of cells; green fluorescence represents the oxidation state of cells. Data are presented as means ± SD from three independent experiments. ** p < 0.01, *** p < 0.001 versus the corresponding control group.

Article Snippet: The endometrial cancer cell lines HEC-1-A, AN3CA and KLE were obtained from Servicebio (Wuhan, China).

Techniques: CCK-8 Assay, Positive Control, Flow Cytometry, Fluorescence, Control

Journal: Biomedicines

Article Title: Targeting the FTO-ACSL4 Pathway: A Novel Mechanism for Sanguinarine Chloride-Induced Ferroptosis in Endometrial Cancer

doi: 10.3390/biomedicines14030608

Figure Lengend Snippet: ACSL4 mediates S.C-induced ferroptosis in endometrial cancer cells. ( A , B ) Representative WB showing ACSL4 protein levels in the indicated cell lines. ( C , D ) The viability of indicated cells following a 48 h treatment with 1.0 μM S.C was determined by the CCK-8 assay. ( E , F ) WB analysis of FTH and ACSL4 protein levels in HEC-1-A and AN3CA cells co-treated with 1.0 μM S.C and 30 μM Berberine for 48 h. ( G , H ) Lipid peroxidation was assessed in HEC-1-A and AN3CA cells following a 48 h concomitant treatment with 1.0 μM S.C and 30 μM Berberine, using the C11-BODIPY 581/591 probe. Data are presented as means ± SD from three independent experiments. ** p < 0.01, *** p < 0.001 versus the corresponding control group.

Article Snippet: The endometrial cancer cell lines HEC-1-A, AN3CA and KLE were obtained from Servicebio (Wuhan, China).

Techniques: CCK-8 Assay, Control

Journal: Biomedicines

Article Title: Targeting the FTO-ACSL4 Pathway: A Novel Mechanism for Sanguinarine Chloride-Induced Ferroptosis in Endometrial Cancer

doi: 10.3390/biomedicines14030608

Figure Lengend Snippet: S.C suppresses FTO to upregulate ACSL4 and to promote lipid peroxidation. ( A , B ) WB analysis of protein levels of ALKBH5, METTL3, USP15, FTO, SENP1, and TRIM21 in HEC-1-A and AN3CA cells treated with 0, 0.5, or 1.0 μM S.C for 48 h. ( C ) Analysis of ACSL4 and FTO protein expression by WB in FTO-overexpressing cells with or without 1.0 μM S.C treatment. ( D , E ) Lipid peroxidation was evaluated in FTO-overexpressing cells following treatment with 1.0 μM S.C or under control conditions, using the C11-BODIPY 581/591 probe.

Article Snippet: The endometrial cancer cell lines HEC-1-A, AN3CA and KLE were obtained from Servicebio (Wuhan, China).

Techniques: Expressing, Control

Journal: Biomedicines

Article Title: Targeting the FTO-ACSL4 Pathway: A Novel Mechanism for Sanguinarine Chloride-Induced Ferroptosis in Endometrial Cancer

doi: 10.3390/biomedicines14030608

Figure Lengend Snippet: S.C inhibits endometrial tumor growth by triggering ferroptosis in vivo. ( A ) Mice bearing HEC-1-A xenografts were treated intraperitoneally with S.C or PBS once every two days ( n = 6 in each group) for a total of 14 days. ( B ) Tumors were harvested and photographed. ( C ) Tumor weights were measured and compared across groups. ( D , E ) Representative images of H&E staining and IHC staining for Ki67 are presented. ( F ) Protein expression levels of ACSL4, FTH, FTL, NCOA4, NRF2, and GPX4 were assessed by WB analysis. Data are presented as means ± SD. * p < 0.05; n.s, not significant.

Article Snippet: The endometrial cancer cell lines HEC-1-A, AN3CA and KLE were obtained from Servicebio (Wuhan, China).

Techniques: In Vivo, Staining, Immunohistochemistry, Expressing